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Characterization of Alpha Amylase and Invertase Using Polyacrylamide Gel Electrophoresis (PAGE) Practical

Title: Characterization of Alpha Amylase and Invertase Using Polyacrylamide Gel Electrophoresis (PAGE) Aim: To characterize alpha amylase and invertase enzymes using Polyacrylamide Gel Electrophores…

Extraction of Papain from Papaya Leaves and Estimation of Its Activity in Terms of Ammonia Practical

Title: Extraction of Papain from Papaya Leaves and Estimation of Its Activity in Terms of Ammonia Aim: To extract papain from papaya leaves and estimate its activity by measuring the amount of amm…

Assay of Alpha Amylase in Terms of Reducing Sugar Produced and Calculation of Enzyme Unit Practical

Title: Assay of Alpha Amylase in Terms of Reducing Sugar Produced and Calculation of Enzyme Unit Aim: To determine the activity of alpha amylase by measuring the amount of reducing sugars produced a…

Partial Purification of Alpha Amylase by Salt Precipitation Method Practical

Title: Partial Purification of Alpha Amylase by Salt Precipitation Method Aim: To partially purify alpha amylase enzyme using salt precipitation method. Introduction:   Alpha amylase is an industri…

Production of Bacterial or Fungal Alpha Amylase Practical

Title: Production of Bacterial or Fungal Alpha Amylase Aim: To study the production of alpha amylase by bacterial or fungal cultures through fermentation. Introduction:   Alpha amylase is an impo…

Study of Substrate Specificity of Enzymes: Practical

Title: Study of Substrate Specificity of Enzymes Aim: To investigate the substrate specificity of an enzyme by testing its activity on different substrates and measuring the rate of reaction. Int…

Assay of Alkaline Phosphatase in Human Serum Practical

Title: Assay of Alkaline Phosphatase in Human Serum Aim: To determine the activity of alkaline phosphatase (ALP) in human serum using a spectrophotometric method. Introduction:   Alkaline phosphata…
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